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lambda ladder pfge marker  (New England Biolabs)


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    Structured Review

    New England Biolabs lambda ladder pfge marker
    Staphylococcus pseudintermedius digested with Apal: The figure shows the staphylococcal cassette mec type and pulsed-field gel electrophoresis pattern of S. pseudintermedius isolates from various sources in the Small Animal Teaching Hospital; G = Gynecology room, IM = Internal Medicine room, P = Post-surgery, and N/A = not detected.
    Lambda Ladder Pfge Marker, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 95/100, based on 808 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pfge+lambda+ladder/Lambda+PFG+Ladder/pmc12913963-117-1-5
    Average 95 stars, based on 808 article reviews
    lambda ladder pfge marker - by Bioz Stars, 2026-09
    95/100 stars

    Images

    1) Product Images from "Molecular epidemiology and environmental persistence of methicillin-resistant coagulase-positive Staphylococci in a Veterinary Teaching Hospital in Thailand: Evidence for nosocomial transmission and One Health implications"

    Article Title: Molecular epidemiology and environmental persistence of methicillin-resistant coagulase-positive Staphylococci in a Veterinary Teaching Hospital in Thailand: Evidence for nosocomial transmission and One Health implications

    Journal: Veterinary World

    doi: 10.14202/vetworld.2025.3698-3712

    Staphylococcus pseudintermedius digested with Apal: The figure shows the staphylococcal cassette mec type and pulsed-field gel electrophoresis pattern of S. pseudintermedius isolates from various sources in the Small Animal Teaching Hospital; G = Gynecology room, IM = Internal Medicine room, P = Post-surgery, and N/A = not detected.
    Figure Legend Snippet: Staphylococcus pseudintermedius digested with Apal: The figure shows the staphylococcal cassette mec type and pulsed-field gel electrophoresis pattern of S. pseudintermedius isolates from various sources in the Small Animal Teaching Hospital; G = Gynecology room, IM = Internal Medicine room, P = Post-surgery, and N/A = not detected.

    Techniques Used: Pulsed-Field Gel, Electrophoresis

    Staphylococcus coagulans digested with cfr9I: The figure shows the staphylococcal cassette mec type and pulsed-field gel electrophoresis patterns of S. coagulans isolates from various sources in the Small Animal Teaching Hospital; G = Gynecology room, IM = Internal Medicine room, ICU = intensive care unit, and H = Hallway.
    Figure Legend Snippet: Staphylococcus coagulans digested with cfr9I: The figure shows the staphylococcal cassette mec type and pulsed-field gel electrophoresis patterns of S. coagulans isolates from various sources in the Small Animal Teaching Hospital; G = Gynecology room, IM = Internal Medicine room, ICU = intensive care unit, and H = Hallway.

    Techniques Used: Pulsed-Field Gel, Electrophoresis

    Related Articles

    Agarose Gel Electrophoresis:

    Article Title: Deletion of a 236 kb region around S 4-RNase in a stylar-part mutant S 4sm-haplotype of Japanese pear.
    Article Snippet: Japanese pear (Pyrus pyrifolia Nakai) has a gametophytic self-incompatibility (GSI) mechanism controlled by a single S-locus with multiple S-haplotypes, each of which contains separate genes that determine the allelic identity of pistil and pollen.. The pistil S gene is the S-ribonuclease (S-RNase) gene, whereas good candidates for the pollen S gene are the F-box protein genes.. A selfcompatible (SC) cultivar, ‘Osa-Nijisseiki’, which is a bud mutant of ‘Nijisseiki’ (S2S4), has a stylar-part mutant S sm 4 haplotype, which lacks the S4-RNase gene but retains the pollen S gene.

    Article Title: Laboratory Stock Variants of the Archetype Silver Resistance Plasmid pMG101 Demonstrate Plasmid Fusion, Loss of Transmissibility, and Transposition of Tn 7 / pco / sil Into the Host Chromosome
    Article Snippet: .. A 3mm slice of each plug was then loaded to a 1% PFGE grade agarose gel (100ml 1 X TAE buffer) along with PFGE lambda ladder (New England Biolabs, United States). ..

    Comparison:

    Article Title: Deletion of a 236 kb region around S 4-RNase in a stylar-part mutant S 4sm-haplotype of Japanese pear.
    Article Snippet: Japanese pear (Pyrus pyrifolia Nakai) has a gametophytic self-incompatibility (GSI) mechanism controlled by a single S-locus with multiple S-haplotypes, each of which contains separate genes that determine the allelic identity of pistil and pollen.. The pistil S gene is the S-ribonuclease (S-RNase) gene, whereas good candidates for the pollen S gene are the F-box protein genes.. A selfcompatible (SC) cultivar, ‘Osa-Nijisseiki’, which is a bud mutant of ‘Nijisseiki’ (S2S4), has a stylar-part mutant S sm 4 haplotype, which lacks the S4-RNase gene but retains the pollen S gene.

    Article Title: Related polymorphic F-box protein genes between haplotypes clustering in the BAC contig sequences around the S-RNase of Japanese pear
    Article Snippet: .. Insert size was estimated by comparison with a PFGE lambda ladder (New England Biolabs, http://www.neb.com/ ). ..

    Article Title: Construction, characterization, and preliminary BAC-end sequencing analysis of a bacterial artificial chromosome library of white clover (Trifolium repens L.).
    Article Snippet: White clover (Trifolium repens L.) is a forage legume widely used in combination with grass in pastures because of its ability to fix nitrogen.. We have constructed a bacterial artificial chromosome (BAC) library of an advanced breeding line of white clover.. The library contains 37 248 clones with an average insert size of approximately 85 kb, representing an approximate 3-fold coverage of the white clover genome based on an estimated genome size of 960 Mb.

    Electrophoresis:

    Article Title: Molecular analysis and distribution of multidrug-resistant Enterococcus faecium isolates belonging to clonal complex 17 in a tertiary care center in Mexico City
    Article Snippet: Briefly, the samples were digested with 50 U of SmaI (New England Biolab, Ipswich, MA, USA) for 4 h at 25°C. .. The digested plugs were separated via electrophoresis in 1% agarose gels (BioRad, Hercules, California, USA) using ultra-pure DNA agarose (BioRad, Hercules, California, USA), with 0.5X TBE as the running buffer in the CHEF MAPPER system (BioRad Laboratories, Hercules, California, USA), run at 6 V/cm at 14°C under two different linear ramped pulse times: 1 to 10 s for 16 h and 10 to 40 s for 22 h. A PFGE lambda ladder (New England Biolabs, Hertfordshire, England, UK) was used as a molecular weight marker, and the gels were stained for 40 m with 0.5 mg/ml of ethidium bromide for visualization under UV light. ..

    Molecular Weight:

    Article Title: Molecular analysis and distribution of multidrug-resistant Enterococcus faecium isolates belonging to clonal complex 17 in a tertiary care center in Mexico City
    Article Snippet: Briefly, the samples were digested with 50 U of SmaI (New England Biolab, Ipswich, MA, USA) for 4 h at 25°C. .. The digested plugs were separated via electrophoresis in 1% agarose gels (BioRad, Hercules, California, USA) using ultra-pure DNA agarose (BioRad, Hercules, California, USA), with 0.5X TBE as the running buffer in the CHEF MAPPER system (BioRad Laboratories, Hercules, California, USA), run at 6 V/cm at 14°C under two different linear ramped pulse times: 1 to 10 s for 16 h and 10 to 40 s for 22 h. A PFGE lambda ladder (New England Biolabs, Hertfordshire, England, UK) was used as a molecular weight marker, and the gels were stained for 40 m with 0.5 mg/ml of ethidium bromide for visualization under UV light. ..

    Article Title: Virulence traits in Cronobacter species isolated from different sources.
    Article Snippet: Cronobacter spp. (Enterobacter sakazakii) includes gram-negative opportunistic foodborne pathogens known as rare but important causes of life-threatening neonatal infections.. However, the pathogenic mechanism is not yet clear.. In this study, 43 isolates of Cronobacter, from human and nonhuman sources, were analyzed.

    Marker:

    Article Title: Molecular analysis and distribution of multidrug-resistant Enterococcus faecium isolates belonging to clonal complex 17 in a tertiary care center in Mexico City
    Article Snippet: Briefly, the samples were digested with 50 U of SmaI (New England Biolab, Ipswich, MA, USA) for 4 h at 25°C. .. The digested plugs were separated via electrophoresis in 1% agarose gels (BioRad, Hercules, California, USA) using ultra-pure DNA agarose (BioRad, Hercules, California, USA), with 0.5X TBE as the running buffer in the CHEF MAPPER system (BioRad Laboratories, Hercules, California, USA), run at 6 V/cm at 14°C under two different linear ramped pulse times: 1 to 10 s for 16 h and 10 to 40 s for 22 h. A PFGE lambda ladder (New England Biolabs, Hertfordshire, England, UK) was used as a molecular weight marker, and the gels were stained for 40 m with 0.5 mg/ml of ethidium bromide for visualization under UV light. ..

    Staining:

    Article Title: Molecular analysis and distribution of multidrug-resistant Enterococcus faecium isolates belonging to clonal complex 17 in a tertiary care center in Mexico City
    Article Snippet: Briefly, the samples were digested with 50 U of SmaI (New England Biolab, Ipswich, MA, USA) for 4 h at 25°C. .. The digested plugs were separated via electrophoresis in 1% agarose gels (BioRad, Hercules, California, USA) using ultra-pure DNA agarose (BioRad, Hercules, California, USA), with 0.5X TBE as the running buffer in the CHEF MAPPER system (BioRad Laboratories, Hercules, California, USA), run at 6 V/cm at 14°C under two different linear ramped pulse times: 1 to 10 s for 16 h and 10 to 40 s for 22 h. A PFGE lambda ladder (New England Biolabs, Hertfordshire, England, UK) was used as a molecular weight marker, and the gels were stained for 40 m with 0.5 mg/ml of ethidium bromide for visualization under UV light. ..

    BAC Assay:

    Article Title: Construction, characterization, and preliminary BAC-end sequencing analysis of a bacterial artificial chromosome library of white clover (Trifolium repens L.).
    Article Snippet: White clover (Trifolium repens L.) is a forage legume widely used in combination with grass in pastures because of its ability to fix nitrogen.. We have constructed a bacterial artificial chromosome (BAC) library of an advanced breeding line of white clover.. The library contains 37 248 clones with an average insert size of approximately 85 kb, representing an approximate 3-fold coverage of the white clover genome based on an estimated genome size of 960 Mb.

    Clone Assay:

    Article Title: Construction, characterization, and preliminary BAC-end sequencing analysis of a bacterial artificial chromosome library of white clover (Trifolium repens L.).
    Article Snippet: White clover (Trifolium repens L.) is a forage legume widely used in combination with grass in pastures because of its ability to fix nitrogen.. We have constructed a bacterial artificial chromosome (BAC) library of an advanced breeding line of white clover.. The library contains 37 248 clones with an average insert size of approximately 85 kb, representing an approximate 3-fold coverage of the white clover genome based on an estimated genome size of 960 Mb.



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    Image Search Results


    Staphylococcus pseudintermedius digested with Apal: The figure shows the staphylococcal cassette mec type and pulsed-field gel electrophoresis pattern of S. pseudintermedius isolates from various sources in the Small Animal Teaching Hospital; G = Gynecology room, IM = Internal Medicine room, P = Post-surgery, and N/A = not detected.

    Journal: Veterinary World

    Article Title: Molecular epidemiology and environmental persistence of methicillin-resistant coagulase-positive Staphylococci in a Veterinary Teaching Hospital in Thailand: Evidence for nosocomial transmission and One Health implications

    doi: 10.14202/vetworld.2025.3698-3712

    Figure Lengend Snippet: Staphylococcus pseudintermedius digested with Apal: The figure shows the staphylococcal cassette mec type and pulsed-field gel electrophoresis pattern of S. pseudintermedius isolates from various sources in the Small Animal Teaching Hospital; G = Gynecology room, IM = Internal Medicine room, P = Post-surgery, and N/A = not detected.

    Article Snippet: A Lambda Ladder PFGE marker (New England BioLabs, USA) served as a size standard.

    Techniques: Pulsed-Field Gel, Electrophoresis

    Staphylococcus coagulans digested with cfr9I: The figure shows the staphylococcal cassette mec type and pulsed-field gel electrophoresis patterns of S. coagulans isolates from various sources in the Small Animal Teaching Hospital; G = Gynecology room, IM = Internal Medicine room, ICU = intensive care unit, and H = Hallway.

    Journal: Veterinary World

    Article Title: Molecular epidemiology and environmental persistence of methicillin-resistant coagulase-positive Staphylococci in a Veterinary Teaching Hospital in Thailand: Evidence for nosocomial transmission and One Health implications

    doi: 10.14202/vetworld.2025.3698-3712

    Figure Lengend Snippet: Staphylococcus coagulans digested with cfr9I: The figure shows the staphylococcal cassette mec type and pulsed-field gel electrophoresis patterns of S. coagulans isolates from various sources in the Small Animal Teaching Hospital; G = Gynecology room, IM = Internal Medicine room, ICU = intensive care unit, and H = Hallway.

    Article Snippet: A Lambda Ladder PFGE marker (New England BioLabs, USA) served as a size standard.

    Techniques: Pulsed-Field Gel, Electrophoresis

    Dendrogram of the PFGE profiles of A. baumannii isolates with regard to MLST analysis (STs). Seven clusters (A-G), each marked with different coloured lines and squares, were identified using a cut-off value above 80% for the similarity coefficient. Analysis was performed by bionumerics software.

    Journal: Scientific Reports

    Article Title: Genotyping of Acinetobacter baumannii clinical isolates from Polish hospitals uncovers novel sequence types

    doi: 10.1038/s41598-025-23877-0

    Figure Lengend Snippet: Dendrogram of the PFGE profiles of A. baumannii isolates with regard to MLST analysis (STs). Seven clusters (A-G), each marked with different coloured lines and squares, were identified using a cut-off value above 80% for the similarity coefficient. Analysis was performed by bionumerics software.

    Article Snippet: DNA size was estimated using the Lambda PFGE Ladder (New England Biolabs, MA, USA).

    Techniques: Software

    Pulsed-field gel electrophoresis (PFGE) Xba I patterns of S. enteritidis isolates with resistance to ciprofloxacin. Cluster analysis and band-matching applications within the BioNumerics Software 7.6 (Applied-Maths, Kortrijk, Belgium) were utilized to evaluate the similarity of PFGE patterns. BS, Baoshan District; JS, Jinshan District; JA, Jianan District; JD Jiading District; LW, Luwan District; MH, Minghang District; HK, Hongkou District; CN, Changning District; PT, Putuo district; PDX, Pudong District.

    Journal: Foods

    Article Title: Clonal Spread and Genetic Mechanisms Underpinning Ciprofloxacin Resistance in Salmonella enteritidis

    doi: 10.3390/foods14020289

    Figure Lengend Snippet: Pulsed-field gel electrophoresis (PFGE) Xba I patterns of S. enteritidis isolates with resistance to ciprofloxacin. Cluster analysis and band-matching applications within the BioNumerics Software 7.6 (Applied-Maths, Kortrijk, Belgium) were utilized to evaluate the similarity of PFGE patterns. BS, Baoshan District; JS, Jinshan District; JA, Jianan District; JD Jiading District; LW, Luwan District; MH, Minghang District; HK, Hongkou District; CN, Changning District; PT, Putuo district; PDX, Pudong District.

    Article Snippet: A phage Lambda PFGE ladder (New England BioLabs, Ipswich, MA, USA) was used as the DNA size marker.

    Techniques: Pulsed-Field Gel, Electrophoresis, Software

    Plasmid profiles of ciprofloxacin-resistant S. enteritidis isolates and corresponding transconjugants determined by S1-PFGE. Lane M, A phage lambda ladder used as a molecular size marker with different bands labeled; lane 1, isolate SJTUF11851; lane 2, isolate SJTUF11851-TC; lane 3, isolate SJTUF12065; lane 4, isolate SJTUF12065-TC; lane 5, isolate SJTUF12074; lane 6, isolate SJTUF12092; lane 7, isolate SJTUF12074-TC; lane 8, isolate SJTUF12092-TC; lane 9, isolate SJTUF12122; lane 10, isolate SJTUF12519; lane 11, isolate SJTUF12122-TC; lane 12, SJTUF12519-TC.

    Journal: Foods

    Article Title: Clonal Spread and Genetic Mechanisms Underpinning Ciprofloxacin Resistance in Salmonella enteritidis

    doi: 10.3390/foods14020289

    Figure Lengend Snippet: Plasmid profiles of ciprofloxacin-resistant S. enteritidis isolates and corresponding transconjugants determined by S1-PFGE. Lane M, A phage lambda ladder used as a molecular size marker with different bands labeled; lane 1, isolate SJTUF11851; lane 2, isolate SJTUF11851-TC; lane 3, isolate SJTUF12065; lane 4, isolate SJTUF12065-TC; lane 5, isolate SJTUF12074; lane 6, isolate SJTUF12092; lane 7, isolate SJTUF12074-TC; lane 8, isolate SJTUF12092-TC; lane 9, isolate SJTUF12122; lane 10, isolate SJTUF12519; lane 11, isolate SJTUF12122-TC; lane 12, SJTUF12519-TC.

    Article Snippet: A phage Lambda PFGE ladder (New England BioLabs, Ipswich, MA, USA) was used as the DNA size marker.

    Techniques: Plasmid Preparation, Marker, Labeling